Table of Contents
1. INTRODUCTION................................................................................................................................................................1-1
I
NSTRUMENT
D
ESCRIPTION
................................................................................................................................................... 1-1
I
NSTRUMENT
S
PECIFICATIONS
............................................................................................................................................... 1-1
Patents...............................................................................................................................................................................1-1
S
AMPLE
R
ETENTION
P
EDESTAL
M
EASUREMENTS
................................................................................................................. 1-2
Pedestal Basic Use............................................................................................................................................................1-2
C
UVETTE
M
EASUREMENTS
.................................................................................................................................................... 1-3
Cuvette Basic Use............................................................................................................................................................. 1-3
B
LANKING AND
A
BSORBANCE
C
ALCULATIONS
..................................................................................................................... 1-4
F
LUORESCENT
D
YES
............................................................................................................................................................. 1-5
2. SOFTWARE.........................................................................................................................................................................2-1
C
OMPUTER
R
EQUIREMENTS
.................................................................................................................................................. 2-1
S
OFTWARE
I
NSTALLATION
.................................................................................................................................................... 2-1
Registering Your Instrument............................................................................................................................................. 2-2
S
OFTWARE
F
EATURES
........................................................................................................................................................... 2-2
Left Pane........................................................................................................................................................................... 2-2
Right Pane......................................................................................................................................................................... 2-5
D
ATA AND
A
CCOUNT
M
ANAGEMENT
.................................................................................................................................... 2-7
My Data ............................................................................................................................................................................ 2-7
Reports.............................................................................................................................................................................. 2-7
Autosave files.................................................................................................................................................................... 2-9
Options..............................................................................................................................................................................2-9
3. APPLICATIONS.................................................................................................................................................................. 3-1
O
VERVIEW
............................................................................................................................................................................ 3-1
Q
UICK
S
TART
........................................................................................................................................................................ 3-1
M
EASUREMENT
R
ANGES
....................................................................................................................................................... 3-2
N
UCLEIC
A
CID
...................................................................................................................................................................... 3-3
Overview........................................................................................................................................................................... 3-3
Nucleic Acid Calculations ................................................................................................................................................3-3
Unique Screen Features ....................................................................................................................................................3-4
Making Nucleic Acid Measurements................................................................................................................................3-5
Oligo Calc.........................................................................................................................................................................3-6
M
ICRO
A
RRAY
...................................................................................................................................................................... 3-8
Overview........................................................................................................................................................................... 3-8
Dye/Chromophore Editor.................................................................................................................................................. 3-8
Unique Screen Features ....................................................................................................................................................3-8
Making Micro Array Measurements.................................................................................................................................3-9
Oligo Calc.......................................................................................................................................................................3-10
UV-V
IS
............................................................................................................................................................................... 3-13
Overview......................................................................................................................................................................... 3-13
Unique Screen Features ..................................................................................................................................................3-13
Making UV-Vis Measurements ...................................................................................................................................... 3-14
P
ROTEIN
A280 ....................................................................................................................................................................3-16
Overview......................................................................................................................................................................... 3-16
Unique Screen Features ..................................................................................................................................................3-17
Making Protein A280 Measurements.............................................................................................................................. 3-18
P
ROTEINS
&
L
ABELS
........................................................................................................................................................... 3-20
Overview......................................................................................................................................................................... 3-20
Dye/Chromophore Editor................................................................................................................................................ 3-20
Unique Screen Features ..................................................................................................................................................3-21
Making Proteins & Labels Measurements...................................................................................................................... 3-22
P
ROTEIN
BCA..................................................................................................................................................................... 3-24
Overview......................................................................................................................................................................... 3-24
Unique Screen Features ..................................................................................................................................................3-25
Making BCA Assay Measurements................................................................................................................................3-27
P
ROTEIN
L
OWRY
................................................................................................................................................................. 3-29
Overview......................................................................................................................................................................... 3-29